Direct RNA Sequencing

Sequence RNA with our Direct RNA Sequencing service. Using Nanopore Sequencing, our service yields native RNA sequence data while preserving base modifications.

This service is highly recommended for researchers who:

Would like to remove RT and PCR bias
Have transcripts that are difficult to reverse transcribe
Are exploring attributes of native RNA such as modified bases

Based on the Direct RNA Sequencing Kit (Oxford Nanopore Technologies)

This service yields:

Direct RNA sequence
Preservation of base modifications

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Speak to a Specialist

Price is determined based on number of samples provided at a time, referred to as batch size.

Create Your Wasatch BioLabs Account
Input Your Sample Details
Review Your Pricing Summary
Confirm Order & Initiate Payment
Download Provided Shipping Labels & Review Handling Instructions
Monitor Sample(s) Status Via Your Portal And/Or Email Notifications
View Results Within 7 Days Of The Lab Receiving The Samples

FAQ

How much RNA should I send?

Please send 400 ng of poly(A)-tailed RNA or 1200 ng of total RNA in 20 µl.

What QC metrics do my samples need to meet?

We run key QC analyses on all received samples. However, to minimize lost time and unnecessary shipping and QC charges, we recommend confirming you meet the following metrics before sending your sample:

RIN/RQN: > 7.0 (> 8.0 preferred)
260/280 ratio: 1.8 - 2.0
260/230 ratio: 1.9 - 2.5

How do I ship my samples?

Detailed shipping instructions will be provided via email once your order has been placed:

Samples will need to be loaded in a 96-well plate in the plate position indicated during the ordering process.
Your plate(s) will need to be labeled with your Order ID number.
Prepaid shipping label(s) will be provided to you to print.
Samples will need to be shipped with 10 lbs of dry ice.

How do I track my samples?

The status of your samples can be found within your Wasatch BioLabs portal, where you will be able to view both individual and/or batch sample status. You can also opt-in to email notifications in the app to receive updates in real-time.

How long will it take to get my data after mailing my samples?

Your samples will arrive at the lab within 2 days of you sending them via USPS Priority Express Shipping. Once received, you will have access to the sample data within 5-7 days via your portal.

How will my data be delivered?

Sample data can be directly downloaded from your WBL portal in .fastq format. You can also set up auto-delivery to receive your data via email once it is ready.

What kit should I use for RNA extraction?

Any RNA extraction kit that can achieve the quality standards above can be used.

What data will I receive?

Results will be returned in .fastq format—the industry standard for sequencing data. To support download performance, the data is split across multiple files and gzipped. For more information on .fastq format and how to combine files, email us at support@wasatchbiolabs.com.