PCR cDNA Sequencing

Sequence the transcriptome with our PCR cDNA Sequencing service.

This service is recommended for researchers who:

Have a limited amount of input material
Wish to multiplex samples to reduce price per sample
Want to optimize their sequencing experiment for output
Would like to identify and quantify full-length transcripts
Are interested in differential gene expression
Want to characterize and quantify isoforms, splice variants and fusion transcripts

Based on the PCR-cDNA barcoding Kit (Oxford Nanopore Technologies)

This service yields:

Identification and quantification of full-length transcripts
Barcodes for up to 24 samples

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Speak to a Specialist

Price is determined based on number of samples provided at a time, referred to as batch size.

Create Your Wasatch BioLabs Account
Input Your Sample Details
Review Your Pricing Summary
Confirm Order & Initiate Payment
Download Provided Shipping Labels & Review Handling Instructions
Monitor Sample(s) Status Via Your Portal And/Or Email Notifications
View Results Within 7 Days Of The Lab Receiving The Samples

FAQ

How much RNA should I send?

Please send 10 ng of poly(A)+ RNA or 500 ng of total RNA.

What QC metrics do my samples need to meet?

We run key QC analyses on all received samples. However, to minimize lost time and unnecessary shipping and QC charges, we recommend confirming you meet the following metrics before sending your sample:

Concentration: > 25 ng/µl by Qubit or similar analysis
RIN/RQN: > 7.0 (> 8.0 preferred)
260/280 ratio: 1.8 - 2.0
260/230 ratio: 1.9 - 2.5

How do I ship my samples?

Detailed shipping instructions will be provided via email once your order has been placed:

Samples will need to be loaded in a 96-well plate in the plate position indicated during the ordering process.
Your plate(s) will need to be labeled with your Order ID number.
Prepaid shipping label(s) will be provided to you to print.
Samples will need to be shipped with 10 lbs of dry ice.

How do I track my samples?

The status of your samples can be found within your Wasatch BioLabs portal, where you will be able to view both individual and/or batch sample status. You can also opt-in to email notifications in the app to receive updates in real-time.

How long will it take to get my data after mailing my samples?

Your samples will arrive at the lab within 2 days of you sending them via USPS Priority Express Shipping. Once received, you will have access to the sample data within 5-7 days via your portal.

How will my data be delivered?

Sample data can be directly downloaded from your WBL portal in .fastq format. You can also set up auto-delivery to receive your data via email once it is ready.

What kit should I use for RNA extraction?

Any RNA extraction kit that can achieve the quality standards above can be used.

What data will I receive?

Results will be returned in .fastq format—the industry standard for sequencing data. To support download performance, the data is split across multiple files and gzipped. For more information on .fastq format and how to combine files, email us at support@wasatchbiolabs.com.