Whole Genome Sequencing - Human Species

Sequence human whole genome with our Whole Genome Sequencing - Human Species Service. Using Oxford Nanopore technology, our sequencing yields native DNA sequence as well as methylation and hydroxymethylation data across the genome.

This service is recommended for researchers who:

Want to optimize their sequencing experiment for output
Wish to sequence 2-3 samples for Whole Genome Sequencing (WGS)
Need a PCR-free method of multiplexing to preserve additional information, such as base modifications
Require control over read length
Would like to utilize upstream processes, such as size selection or whole genome amplification
Would like to process multiple samples simultaneously, either with a multichannel pipette or a liquid handling robot

Based on the Multiplex Ligation Sequencing Kit (Oxford Nanopore Technologies)

This service yields:

Native, PCR-free DNA sequence
Methylation and hydroxymethylation status
Copy number variation

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Speak to a Specialist

Price is determined based on number of samples provided at a time, referred to as batch size.

Create Your Wasatch BioLabs Account
Input Your Sample Details
Review Your Pricing Summary
Confirm Order & Initiate Payment
Download Provided Shipping Labels & Review Handling Instructions
Monitor Sample(s) Status Via Your Portal And/Or Email Notifications
View Results Within 7 Days Of The Lab Receiving The Samples

FAQ

How much DNA should I send?

Please send 1000 ng of DNA in 25 µl of water.

What QC metrics do my samples need to meet?

We run key QC analyses on all received samples. However, to minimize lost time and unnecessary shipping and QC charges, we recommend confirming you meet the following metrics before sending your sample:

Concentration: 16 ng/µl by Qubit or similar analysis
260/280 ratio: 1.8 - 2.0
260/230 ratio: 1.9 - 2.5
High molecular weight band on gel, or better, Pulse Field Electrophoresis results
Showing average fragment sizes > 100 kb

How do I ship my samples?

Detailed shipping instructions will be provided via email once your order has been placed:

Samples will need to be loaded in a 96-well plate in the plate position indicated during the ordering process.
Your plate(s) will need to be labeled with your Order ID number.
Prepaid shipping label(s) will be provided to you to print.
Samples will need to be shipped with 10 lbs of dry ice.

How do I track my samples?

The status of your samples can be found within your Wasatch BioLabs portal, where you will be able to view both individual and/or batch sample status. You can also opt-in to email notifications in the app to receive updates in real-time.

How long will it take to get my data after mailing my samples?

Your samples will arrive at the lab within 2 days of you sending them via USPS Priority Express Shipping. Once received, you will have access to the sample data within 5-7 days via your portal.

How will my data be delivered?

Sample data can be directly downloaded from your WBL portal in .fastq format. You can also set up auto-delivery to receive your data via email once it is ready.

What kit should I use for extraction?

For cells, blood, or animal tissue, we highly recommend you use the NEB HMW Extraction Kit, as it consistently provides the best results. For plants and difficult-to-lyse animal tissues (e.g., sea sponge), we recommend the Promega Wizard HMW Extraction Kit.

What data will I receive?

Results will be returned in .fastq format—the industry standard for sequencing data. To support download performance, the data is split across multiple files and gzipped. For more information on .fastq format and how to combine files, email us at support@wasatchbiolabs.com.