cDNA Sequencing Services

Full-Length cDNA Transcript Sequencing

Capture full-length transcript diversity with WBL's cDNA sequencing service, powered by Oxford Nanopore Technologies (ONT). This service provides detailed insights into gene expression, alternative splicing, and isoform diversity by sequencing the entire cDNA length, enabling a deeper understanding of transcriptomic landscapes and gene regulation.

The Power of Long read sequencing

Capture the full transcriptomic landscape across diverse species, resolving highly repetitive and other complex regions.

cDNA

Short Read RNA

ONT Direct RNA

RNA Sequencing

Small Nucleotide Variants

Methylation & Hydroxy Methylation

Structural Variants

Copy Number Variation

Repetitive Regions

Haplotype

Splice Variants

Fusion Transcripts

Accepted RNA Sample Sources:

Humans

Animals

Plants

Bacteria*

Virus*

Accepted RNA Sample Types:

Poly(A)+ RNA

Total RNA

*WBL currently only accepts DNA and RNA that are extracted from samples that are not classified as infectious. This policy ensures the safety of our laboratory personnel and compliance with regulatory standards. Please ensure that all samples are appropriately treated and certified as non-infectious before submission. If you have any questions about sample preparation or our safety standards, please contact our support team at: support@wasatchbiolabs.com.

More Than a Service Provider — Your Trusted Partner

Accelerate your research with comprehensive nanopore data. With native precision and long reads, Wasatch BioLabs' Nanopore services deliver actionable insights with confidence in every read.

ONT Certified Precision

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Leverage Oxford Nanopore Technologies-certified services, supported by rigorous quality assurance and control metrics, to achieve reliable data for your research.

Bioinformatic Expertise

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Enhance your nanopore data with custom bioinformatics solutions, using tailored workflows and advanced analysis to generate impactful, publication-ready insights.

One-On-One Collaboration

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Work directly with sequencing and bioinformatics experts who provide personalized, end-to-end support, guiding you from project planning to data interpretation.

FAQ

What is cDNA sequencing, and how does it differ from direct RNA (dRNA) sequencing?

cDNA sequencing involves reverse transcribing RNA into complementary DNA prior to sequencing, whereas direct RNA sequencing analyzes native RNA molecules without conversion. Direct RNA sequencing preserves RNA modifications, while cDNA sequencing offers higher throughput and improved error correction, making it well suited for large-scale gene expression and transcript diversity studies.

What are the advantages of Wasatch BioLabs' cDNA sequencing compared to other providers?

Wasatch BioLabs combines Oxford Nanopore long-read sequencing with expert bioinformatics support to deliver high-quality, full-length transcript data. Our approach enables accurate isoform detection, alternative splicing analysis, and transcript structure resolution beyond the limits of short-read sequencing.

What are the sample requirements for Wasatch BioLabs’ cDNA sequencing services?

Wasatch BioLabs accepts high-quality total RNA or poly(A)+ RNA from a wide range of non-infectious sources, including human, animal, plant, bacterial, and viral samples. All samples must be certified as non-infectious prior to submission. Questions regarding sample quality, preparation, or shipment can be directed to our team at support@wasatchbiolabs.com.

How does Wasatch BioLabs address RNA quality and potential degradation in cDNA sequencing?

We apply quality control checks to assess RNA integrity prior to reverse transcription. If degradation is detected, our team provides guidance on sample re-preparation or workflow optimization to ensure cDNA libraries accurately reflect transcript content and minimize downstream artifacts.

Does Wasatch BioLabs offer bioinformatics support for cDNA data?

Yes, we offer comprehensive bioinformatics support for cDNA sequencing data, including custom analysis workflows and one-on-one collaboration to support data interpretation and generation of publication-ready insights. Analysis options include isoform quantification, splice variant detection, expression profiling, and fusion transcript identification.

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