[from Development to release]

Confidence In Every Molecule

Wasatch BioLabs’ Oxford Nanopore mRNA Vaccine QC service provides complete construct validation in a single assay. Using direct RNA sequencing, we capture full-length mRNA molecules without amplification or reverse transcription, preserving 5′/3′ integrity, poly(A) tail length, and native sequence fidelity. Whether you’re developing novel mRNA constructs or performing lot-release testing, our workflow delivers single-molecule resolution and comprehensive insight across every transcript.

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Streamlining mRNA Quality Control in a Single Workflow

Traditional QC workflows require multiple assays to confirm sequence, integrity, and purity. WBL’s long-read platform consolidates these into a single, rapid, and regulatory-aligned solution.

Single-Assay Resolution:

Simultaneously measures sequence, integrity, and purity.

Native RNA Fidelity:

Sequences unmodified RNA to preserve authentic structure.

Lot-Release Efficiency:

Delivers validated deliverables within seven business days.

Attribute

Traditional QC Methods

WBL Long-Read mRNA QC

Workflow Complexity

Multiple Separate Assays For Sequence, Integrity, and Poly(A) Tails.

Single Assay Measures All Metrics Simultaneously

Amplification Artifacts

Common Due To Reverse Transcription Or PCR

None, Native RNA Is Sequenced Directly

5′/3′ Structure

Often Lost Or Partially Captured

Fully Preserved At Molecule-Level Resolution

Poly(A) Tail Measurement

Indirect Or Estimated VIA Secondary Methods

Direct Peak-Based Measurement Of Tail Length

Data Integration

Fragmented Results, Manual Consolidation Required

Unified Bioinformatics Pipeline With Automated Reporting

Turnaround Time

Typically 2–3 Weeks

Standard 7 Business Days

Scalability

Limited By Disconnected Workflows

Scales Seamlessly From R&D To Lot Release Testing

The Case for Long-Read Sequencing in mRNA QC

Short-read and synthetic assays fragment transcripts, compromising molecule integrity and key features of gene expression. WBL’s long-read mRNA QC directly sequences full-length molecules, capturing every element essential for accurate characterization and product validation.

Sample Submission:
Requires only 300 ng poly(A)+ RNA or 1 µg total RNA (8 µL volume).
Native Sequencing:
Captures full-length transcripts with intact 5′/3′ and poly(A) structures.
Data Processing:
Automates basecalling, contamination detection, and integrity assessment.
QC Report:
Automates basecalling, contamination detection, and integrity assessment.

From Sample to Report: A Streamlined, End-to-End QC Workflow

WBL’s mRNA Vaccine QC assay consolidates multiple traditional tests into one direct-sequencing workflow. With as little as 300 ng of poly(A)-tailed RNA, you’ll receive a full construct assessment, from sequence integrity to poly(A) tail profiling, within 7-10 business days.

Details

Accepted Sample Types

100% Polyadenylated RNA Samples

Multiplexing Capacity

Up To 16 Transcripts Per Sequencing Runs

Turnaround Time

Standard Delivery Within 7 Business Days

Core Outputs

Sequence Integrity, Contamination, Abundance, And Poly(A) Profiles

Deliverables

QC Report, Custom Bioinformatics, Optional Validation And Lot-release

Support

Dedicated Project Manager With Consultative Onboarding

Deliverables & Key Outputs

Sequencing & Contamination

Integrity & Accuracy

Quantity & Poly(A) Profiles

Key Output

Alignment rate, read length, PHRED quality, and contamination levels

Percent intact transcripts and per-transcript mismatch rates

RNA abundance and poly(A) tail length distribution

Beyond QC: Your Partner in RNA Development

Wasatch BioLabs combines native-read sequencing, customizable bioinformatics, and regulatory-ready workflows to accelerate your mRNA vaccine development and clinical validation.

Long-Read Workflows

[Service]

Purpose-built long-read sequencing workflows that validate native RNA constructs with full-length resolution and comprehensive molecular insight.

Integrated Bioinformatics

[Service]

Customizable bioinformatic pipelines providing automated reporting, streamlined analysis, and seamless delivery of all key quality metrics.

Regulatory Alignment

[Service]

GCP- and CLIA-ready processes designed to support validation studies, release testing, and compliant translational program workflows.

SERVICES

mRNA Vaccine QC with Long-Read Sequencing

[from Development to release]

Confidence In Every Molecule

Streamlining mRNA Quality Control in a Single Workflow

Single-Assay Resolution:

Native RNA Fidelity:

Lot-Release Efficiency:

Visualizing The Workflow

Attribute

Traditional QC Methods

WBL Long-Read mRNA QC

The Case for Long-Read Sequencing in mRNA QC

From Sample to Report: A Streamlined, End-to-End QC Workflow

Category

Details

Accepted Sample Types

100% Polyadenylated RNA Samples

Multiplexing Capacity

Up To 16 Transcripts Per Sequencing Runs

Turnaround Time

Standard Delivery Within 7 Business Days

Core Outputs

Sequence Integrity, Contamination, Abundance, And Poly(A) Profiles

Deliverables

QC Report, Custom Bioinformatics, Optional Validation And Lot-release

Support

Dedicated Project Manager With Consultative Onboarding

Deliverables & Key Outputs

Category

Sequencing & Contamination

Integrity & Accuracy

Quantity & Poly(A) Profiles

Key Output

Beyond QC: Your Partner in RNA Development

Long-Read Workflows

[Service]

Integrated Bioinformatics

[Service]

Regulatory Alignment

[Service]

Accelerate and Simplify Your mRNA Vaccine QC

From early development through release, Wasatch BioLabs provides confidence in every molecule with single-assay, long-read mRNA QC, delivering complete sequence, integrity, and purity data in one streamlined workflow.