Direct Targeted Methylation Sequencing (gdna)

WBL's targeted native-read sequencing assay offers a cost-effective, high-throughput method unavailable anywhere else. It’s especially useful when your use case only requires targeting specific genes or regions are of interest. By preserving native DNA modifications and eliminating amplification bias, this approach provides accurate and reliable data for biomarker discovery, clinical assay development, and epigenetic studies.

They are highly customizable as  researchers can define their regions of interest, and Wasatch BioLabs designs a corresponding probe set tailored to those targets. The dTMS workflow adapts to the unique goals of your study, reagardless of whether focusing on specific genes, regulatory elements, or CpG islands.

Direct Targeted Methylation Sequencing is optimized for high-quality genomic DNA (gDNA) samples. The process is particularly effective when intact DNA is available, enabling accurate analysis of sequence and methylation information without the degradation risks associated with harsh treatments like bisulfite conversion.

We make sure that accuracy is maintained through a combination of proprietary library prep, hybrid capture protocols, and long-read native nanopore sequencing. This method avoids common sources of bias, such as PCR amplification or chemical conversion, and delivers single-molecule resolution data — ensuring precise and reproducible results.

Clients receive detailed sequence and methylation data, including information on 5mC and 5hmC levels, phasing, structural variants, and small variant calls. Data access and visualization tools are also provided, and comprehensive bioinformatics support helps translate raw data into actionable insights.

From the start, Wasatch BioLabs works closely with clients to identify target regions and design custom probe sets. The team offers end-to-end support, from experimental planning to final analysis, ensuring that each targeted panel aligns with the research or clinical objectives.