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Direct RNA Sequencing Services

Full-Length Native Transcript Sequencing

Explore isoform-level resolution of the transcriptome with WBL’s Direct RNA Sequencing service. Leveraging Oxford Nanopore Technologies (ONT) Direct RNA Sequencing, WBL’s service enables the direct sequencing of full-length, native RNA transcripts without reverse transcription or amplification biases.

Direct RNA Sequencing allows researchers to sequence the transcriptome and visualize native epigenetic modifications, offering researchers and clinical service providers richer transcriptome data with unprecedented insights for multi-omics studies.

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The Power of Long read sequencing

Capture the full complexity of RNA molecules in their native state. ONT’s long-reads enable identification of full-length transcripts across diverse species, the resolution of highly repetitive and other complex regions, and the direct detection of post-transcriptional modifications.

Explore the advantages of WBL’s Long-Read Direct RNA Sequencing compared to cDNA and short-read RNA sequencing methods:

cDNA

Short Read RNA

ONT Direct RNA

RNA Sequencing

Small Nucleotide Variants

Methylation & Hydroxy Methylation

Structural Variants

Copy Number Variation

Repetitive Regions

Haplotype

Splice Variants

Fusion Transcripts

Direct RNA Sequencing:
Applications & Use Cases

WBL’s Direct RNA Sequencing offering is ideal for the following types of analyses:

Isoform Analysis

Expression Quantification

Analysis of Methylation

Isoform Co-Quantitative Expression

Fusion Gene Identification

5mC Site Annotation

5mC Site Annotation

Differential Enrichment Analysis

IncRNA Analysis

Transcript Difference Analysis

6mA Site Annotation

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PolyA Analysis

Enrichment Analysis

Enrichment Analysis of Methlation Modifications

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Protein Interaction Network

5mC Differential Analysis

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6mA Differential Analysis

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Accepted RNA Source Samples:

Human

Animal

Plant

Bacteria*

Virus*

Accepted RNA Sources Types:

Poly(A)+ RNA

Total RNA

*WBL currently only accepts DNA and RNA that are extracted from samples that are not classified as infectious. This policy ensures the safety of our laboratory personnel and compliance with regulatory standards. Please ensure that all samples are appropriately treated and certified as non-infectious before submission. If you have any questions about sample preparation or our safety standards, please contact our support team at: support@wasatchbiolabs.com.

More Than a Service Provider — Your Trusted Partner

Accelerate your research with comprehensive nanopore data. With native precision and long reads, Wasatch BioLabs' Nanopore services deliver actionable insights with confidence in every read.

ONT Certified Precision

[Service]

Leverage Oxford Nanopore Technologies-certified services, supported by rigorous quality assurance and control metrics, to achieve reliable data for your research.

Bioinformatic Expertise

[Service]

Enhance your nanopore data with custom bioinformatics solutions, using tailored workflows and advanced analysis to generate impactful, publication-ready insights.

One-On-One Collaboration

[Service]

Work directly with sequencing and bioinformatics experts who provide personalized, end-to-end support, guiding you from project planning to data interpretation.

FAQ

What is direct RNA sequencing and how does it differ from traditional RNA sequencing methods?

Direct RNA sequencing is a method that sequences full-length native RNA molecules directly, without the need for reverse transcription or amplification. Unlike traditional cDNA-based or short-read RNA sequencing methods, it preserves original RNA structure and modifications, enabling more accurate analysis of isoforms, splice variants, and epigenetic features like methylation.

How can Wasatch BioLabs’ RNA sequencing services help with transcriptomics studies?

Wasatch BioLabs offers long-read direct RNA sequencing that provides full-length transcript data and captures native RNA modifications. This approach supports high-resolution transcriptomics by enabling isoform-level analysis, expression quantification, alternative splicing identification, and more — delivering deep insights for both research and clinical applications.

What types of RNA expression analysis can be performed using Wasatch BioLabs’ direct RNA sequencing?

Our direct RNA sequencing supports a wide range of expression-related analyses, including isoform quantification, differential gene expression, fusion gene detection, long non-coding RNA analysis, and post-transcriptional modification profiling. It also allows for enrichment analysis and annotation of RNA methylation sites like 5mC and 6mA.

What sample requirements does Wasatch BioLabs have for RNA sequencing projects?

Wasatch BioLabs accepts total RNA or poly(A)+ RNA from a variety of organisms including human, animal, plant, bacteria, and viruses. However, samples must be non-infectious and certified as such prior to submission. If there's uncertainty about sample status or preparation, our support team is available to assist.

What advantages does direct RNA sequencing offer over cDNA-based approaches?

Direct RNA sequencing eliminates the biases introduced during reverse transcription and PCR amplification. It captures native RNA sequences and their modifications, provides longer read lengths for better isoform resolution, and accurately reflects the transcriptome’s true complexity — making it ideal for precise RNA expression studies.

Does Wasatch BioLabs offer bioinformatics support or RNA data analysis services?

Yes, we offer comprehensive bioinformatics support tailored to nanopore RNA data. Our team provides advanced workflows and one-on-one collaboration to help researchers interpret data and generate publication-ready insights.

Specialized offerings in our next-generation Biolab

Explore our other Nanopore Services:

PCR Amplicon sequencing
cDNA sequencing

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