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Direct RNA Sequencing Services

Full-Length Native Transcript Sequencing

Explore isoform-level resolution of the transcriptome with WBL’s Direct RNA Sequencing service. Leveraging Oxford Nanopore Technologies (ONT) Direct RNA Sequencing, WBL’s service enables the direct sequencing of full-length, native RNA transcripts without reverse transcription or amplification biases.

Direct RNA Sequencing allows researchers to sequence the transcriptome and visualize native epigenetic modifications, offering researchers and clinical service providers richer transcriptome data with unprecedented insights for multi-omics studies.

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The Power of Long read sequencing

Capture the full complexity of RNA molecules in their native state. ONT’s long-reads enable identification of full-length transcripts across diverse species, the resolution of highly repetitive and other complex regions, and the direct detection of post-transcriptional modifications.

Explore the advantages of WBL’s Long-Read Direct RNA Sequencing compared to cDNA and short-read RNA sequencing methods:

cDNA

Short Read RNA

ONT Direct RNA

RNA Sequencing

Small Nucleotide Variants

Methylation & Hydroxy Methylation

Structural Variants

Copy Number Variation

Repetitive Regions

Haplotype

Splice Variants

Fusion Transcripts

Direct RNA Sequencing:
Applications & Use Cases

WBL’s Direct RNA Sequencing offering is ideal for the following types of analyses:

Isoform Analysis

Expression Quantification

Analysis of Methylation

Isoform Co-Quantitative Expression

Fusion Gene Identification

5mC Site Annotation

5mC Site Annotation

Differential Enrichment Analysis

IncRNA Analysis

Transcript Difference Analysis

6mA Site Annotation

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PolyA Analysis

Enrichment Analysis

Enrichment Analysis of Methlation Modifications

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Protein Interaction Network

5mC Differential Analysis

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6mA Differential Analysis

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Accepted RNA Source Samples:

Human

Animal

Plant

Bacteria*

Virus*

Accepted RNA Sources Types:

Poly(A)+ RNA

Total RNA

*WBL currently only accepts DNA and RNA that are extracted from samples that are not classified as infectious. This policy ensures the safety of our laboratory personnel and compliance with regulatory standards. Please ensure that all samples are appropriately treated and certified as non-infectious before submission. If you have any questions about sample preparation or our safety standards, please contact our support team at: support@wasatchbiolabs.com.

More Than a Service Provider — Your Trusted Partner

Accelerate your research with comprehensive nanopore data. With native precision and long reads, Wasatch BioLabs' Nanopore services deliver actionable insights with confidence in every read.

ONT Certified Precision

[Service]

Leverage Oxford Nanopore Technologies-certified services, supported by rigorous quality assurance and control metrics, to achieve reliable data for your research.

Bioinformatic Expertise

[Service]

Enhance your nanopore data with custom bioinformatics solutions, using tailored workflows and advanced analysis to generate impactful, publication-ready insights.

One-On-One Collaboration

[Service]

Work directly with sequencing and bioinformatics experts who provide personalized, end-to-end support, guiding you from project planning to data interpretation.

FAQ

What is direct RNA sequencing and how does it differ from traditional RNA-seq methods?

Direct RNA sequencing reads full-length, native RNA molecules directly using Oxford Nanopore technology, without reverse transcription or PCR amplification. Unlike cDNA-based or short-read RNA-seq, it preserves RNA sequence, structure, and chemical modifications, enabling more accurate analysis of isoforms, splice variants, and RNA modifications.

How can direct RNA sequencing service support transcriptomics studies?

Direct RNA sequencing generates full-length transcript data while preserving native RNA modifications. This facilitates isoform-level resolution, accurate splice variant detection, and improved transcript annotation, supporting detailed transcriptomic and epitranscriptomic analysis.

What types of RNA expression analysis can be performed using Wasatch BioLabs’ Direct RNA Sequencing?

Direct RNA sequencing supports a wide range of RNA expression analyses, including isoform-level quantification, differential gene expression, fusion transcript detection, long non-coding RNA characterization, and post-transcriptional modification profiling. The approach also supports identification and annotation of RNA methylation sites, such as 5mC and 6mA, and downstream enrichment analyses.

What are the sample requirements for Wasatch BioLabs’ Direct RNA Sequencing services?

Wasatch BioLabs accepts high-quality total RNA or poly(A)+ RNA from a wide range of organisms, including human, animal, plant, bacterial, and viral sources. All samples must be non-infectious and certified as such prior to submission. Questions regarding sample quality, preparation, or shipment can be directed to our team at support@wasatchbiolabs.com.

What advantages does direct RNA sequencing offer over cDNA-based approaches?

Direct RNA sequencing profiles native RNA molecules without reverse transcription or PCR amplification, eliminating associated biases. This approach preserves RNA modifications, delivers long reads for improved isoform resolution, and more accurately represents transcriptome complexity, making it well suited for RNA expression and isoform analyses.

Does Wasatch BioLabs offer bioinformatics support for Direct RNA data?

Yes, we offer comprehensive bioinformatics support for nanopore RNA data, including custom analysis workflows and one-on-one collaboration with our team to support data interpretation and generation of publication-ready insights.

Specialized offerings in our next-generation Biolab

Explore our other Nanopore Services:

PCR Amplicon sequencing
cDNA sequencing

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